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Assessment of four different detergents used to extract membrane proteins from Xylella fastidiosa by two-dimensional electrophoresis BJM
Di Ciero,Luciana; Bellato,Cláudia de M.; Meinhardt,Lyndel W.; Ferrari,Fernanda; Castellari,Rafael R.; Marangoni,Sérgio; Novello,José C..
Four different detergents, ASB 14, SB 3-10, CHAPS and Triton X100, were utilized to determine the optimal detergent for the solubilization of membrane proteins from the phytopathogenic bacterium Xylella fastidiosa. These proteins were differentially solubilized in distinct buffers containing the detergent and subjected to bidimensional electrophoresis within the non-linear pH range of 3-10. The detergents ASB 14 and SB 3-10 were the most effective revealing 221 and 157 spots, respectively. CHAPS and Triton X100 were less effective and revealed only 72 and 43 spots, respectively. MALDI-TOF tryptic peptide mass fingerprinting of 18 excised proteins from the ASB 14 treatment revealed that 83% were membrane proteins and that the theoretical efficiency of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: X. fastidiosa; 2-DE; Extraction; Proteome; Solubilization.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200018
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Ligninolytic fungus Polyporus sp. S133 mediated metabolic degradation of fluorene BJM
Lazim,Zainab Mat; Hadibarata,Tony.
ABSTRACT This study aimed to investigate the impact of nonionic surfactants on the efficacy of fluorine degradation by Polyporus sp. S133 in a liquid culture. Fluorene was observed to be degraded in its entirety by Polyporus sp. S133 subsequent to a 23-day incubation period. The fastest cell growth rate was observed in the initial 7 days in the culture that was supplemented with Tween 80. The degradation process was primarily modulated by the activity of two ligninolytic enzymes, laccase and MnP. The highest laccase activity was stimulated by the addition of Tween 80 (2443 U/L) followed by mixed surfactant (1766 U/L) and Brij 35 (1655 U/L). UV-vis spectroscopy, TLC analysis and mass spectrum analysis of samples subsequent to the degradation process in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metabolites; Ligninolytic enzymes; Polyporus sp. S133; Non-ionic surfactants; Solubilization.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300610
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Plant- and microbial-based mechanisms to improve the agronomic effectiveness of phosphate rock: a review Anais da ABC (AABC)
Arcand,Melissa M.; Schneider,Kim D..
Deficiency in plant-available phosphorus is considered to be a major limiting factor to food production in many agricultural soils. Mineral resources are necessary to restore soil phosphorus content. In regions where conventional fertilizers are not used due to cost limitations or to mitigate adverse environmental effects, local sources of phosphate rock are being increasingly recognized for potential use as alternative phosphorus fertilizers. The main obstacle associated with using directly applied ground phosphate rock is that the phosphate released is often unable to supply sufficient plant-available phosphorus for crop uptake. Plantand microbial-based mechanisms are low-cost, appropriate technologies to enhance the solubilization and increase the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agriculture; Microorganisms; Phosphate rock; Phosphorus; Rhizosphere; Solubilization.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000400013
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Potencial agrícola de bactérias endofíticas obtidas de bambu Guadua spp. Infoteca-e
VALE, S. M. L. S. do; VALE, P. A. A. do; CARVALHO, C. M.; SIVIERO, A..
Esta pesquisa teve por objetivo caracterizar as bactérias endofíticas de Guadua spp. e estimar o seu potencial de uso agrícola como solubilizados de fosfato e fixação de nitrogênio.
Tipo: Capítulo em livro técnico (INFOTECA-E) Palavras-chave: Sustancias de crecimiento vegetal; Solubilización.; Fijación del nitrógeno; Bactéria endofítica; Endophytic bacteria; Cultivo in vitro; Guadua chaparensis; Guadua weberbeauri; Bambu; Fosfato.; Solubilização; Estimulante de Crescimento Vegetal; Cultura In Vitro; Fixação de Nitrogênio; Biofertilizante; Nitrogen fixation; Phosphates.; Bamboos; Guadua; Plant growth substances; In vitro culture; Solubilization.
Ano: 2019 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1119043
Registros recuperados: 4
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